【佳学基因检测】miR-205在前列腺癌中的表达及与患者预后的关系

国内肿瘤基因检测权威机构名单解密

体会听到《Comput Math Methods Med》在. 2022 Aug 30;2022:1784791.发表了一篇题目为《miR-205在前列腺癌中的表达及与患者预后的关系》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Zhuifeng Guo, Xuwei Lu, Fan Yang, Liang Qin, Ning Yang, Peiran Cai, Conghui Han, Jiawen Wu, Hang Wang 等完成。促进了肿瘤的正确治疗与个性化用药的发展，进一步强调了基因信息检测与分析的重要性。

肿瘤靶向药物及正确治疗临床研究内容关键词：

肿瘤靶向治疗基因检测临床应用结果

目的：我们旨在探讨前列腺癌患者血清和细胞外泌体miR-205水平的变化及其临床意义。材料与方法：首先，利用UALCAN数据库分析miR-205在正常和前列腺癌组织中的表达。 .分析肿瘤组织中miR-205与前列腺癌患者病理及临床特征的关系。因此，于2016年8月至2021年8月在闵行医院收集60例前列腺癌患者。采集两组患者的血清，提取血清外泌体中的RNA，采用qRT-PCR分析miR的表达。 -205 由血清外泌体介导。同时，比较了前列腺癌患者的临床及病理特征与外泌体介导的miR-205表达水平之间的关系。接下来，使用伤口愈合和 CKK-8 等检测方法研究 miR-205 在从前列腺癌中提取的外泌体中对前列腺癌增大和转移的影响。结果：结果表明 miR-205 在组织中的显着水平前列腺癌明显低于正常前列腺组织。此外，miR-205在前列腺癌患者的液体外泌体和来自前列腺癌细胞系的外泌体中的表达水平明显低于健康患者的血清外泌体和前列腺正常细胞系中的表达水平。前列腺癌患者流体外泌体中 miR-205 的表达水平与癌症分期、淋巴结中不受控制的细胞分裂、远处转移和初始诊断时的 PSA 水平呈负相关。分析（多变量和单变量）表明，miR-205 的表达是前列腺癌患者预后的主权威胁原因。此外，miR-205的过表达可以限制前列腺癌的表达和转移。结论：液体来源的外泌体中miR-205的表达与前列腺癌患者的预测相关，可能是一种新的鉴定标志物。和治疗前列腺癌。

肿瘤发生与反复转移国际数据库描述：

Purpose: We aimed to investigate the changes of serum and cell exosome miR-205 levels in patients with prostate carcinoma and its clinical significance.Materials and methods: Firstly, pronouncement of miR-205 in normal and prostate carcinoma tissues was analyzed by using UALCAN database. The relationship between miR-205 in tumor tissues and the pathological and clinical characteristics of patients with prostate carcinoma were analyzed. Consequently, 60 people with prostate carcinoma were collected to the Minhang Hospital from August 2016 to August 2021. Serum of patients in the two groups was collected, and RNA in serum exosomes was extracted, and qRT-PCR was used to analyze the expression of miR-205 mediated by serum exosomes. Meanwhile, the relationship among the clinical as well as pathological aspects and bodement of patients with prostate carcinoma and the pronouncement level of miR-205 mediated by exosome was compared. Next, assays like wound healing and CKK-8 were used to investigate the effects of miR-205 in exosomes extracted from prostate carcinoma on the augmentation and metastasis of prostate carcinoma.Results: The results showed that the pronouncement level of miR-205 in tissues with prostate carcinoma was significantly lower than that in normal prostate tissues. In addition, the pronouncement level of miR-205 in fluid exosome of people with prostate carcinoma and exosomes derived from the lines of prostate carcinoma was considerably less than that in serum exosomes of healthy patients and that of normal cell lines of prostate. The pronouncement level of miR-205 in fluid exosomes of people with prostate carcinoma was negatively associated with cancer phase, uncontrolled cell division in lymph nodes, distant metastasis, and PSA level at initial diagnosis. Analysis (multivariate and univariate) showed that miR-205 pronouncement was a sovereign threat cause for prognosis of prostate cancer patients. Additionally, the pronouncement and metastasis of prostate carcinoma can be restricted by the overexpression of miR-205.Conclusion: The pronouncement of miR-205 in liquid derived exosomes is correlated with the prediction of people with prostate carcinoma and may be a new marker for identification and cure of prostate carcinoma.