【佳学基因检测】腾龙补中颗粒与结肠癌基因检测

小孩发育迟缓查基因有用吗排名

研究dota2吧雷电竞 基因检测中的数据库比对与基因解码了解《J Tradit Chin Med》在. 2022 Oct;42(5):701-706.发表了一篇题目为《腾龙补中颗粒抑制SW620人结肠癌的生长》dota2吧雷电竞 dota2吧雷电竞 治疗基因检测临床研究文章。该研究由L I Miao, Zheng Jialu, Wang Shuangshuang, Chen Lei, Peng Xiao, Chen Jinfang, A N Hongmei, H U Bing等完成。促进了dota2吧雷电竞 的正确治疗与个性化用药的发展，进一步强调了基因信息检测与分析的重要性。

肿瘤靶向药物及正确治疗临床研究内容关键词：

腾龙补中颗粒,血管生成,细胞凋亡,细胞衰老,结肠肿瘤,信号转导

肿瘤靶向治疗基因检测临床应用结果

目的：观察腾龙补中汤颗粒制剂即腾龙补中颗粒（，TBG）对人SW620结肠癌的抗癌作用。用 TBG（2.56 g/kg，每天一次）和/或 5-Fu（104 mg/kg，每周一次）治疗 21 天。通过商业试剂盒测量细胞凋亡、半胱天冬酶活性和细胞衰老。 Western blot或免疫组化检测蛋白表达和磷酸化。结果：TBG和5-Fu抑制肿瘤生长。 TBG、5-Fu、TBG+5-Fu组的抑瘤率分别为42.25%、51.58%、76.08%。 TBG 和 5-Fu 的组合显示出协同抗癌作用。 TBG 和 5-Fu 诱导细胞凋亡，激活 caspase-3、-8 和 -9，增加 SMAC 表达，抑制 XIAP 表达。 TBG 诱导细胞衰老，上调细胞周期蛋白依赖性激酶抑制剂 1a (CDKN1a) 和细胞周期蛋白依赖性激酶抑制剂 2a (CDKN2a) 的表达，并抑制视网膜母细胞瘤相关蛋白 (RB) 的磷酸化和细胞周期蛋白 E1 (CCNE1) 的表达和细胞周期蛋白依赖性2. TBG 还抑制血管生成，同时下调血管内皮生长因子 (VEGF) 和缺氧诱导因子-1α (HIF-1α)。结论：TBG 抑制 SW620 结肠癌生长，诱导细胞凋亡 SMAC-XIAP-Caspase信号通路，通过CDKN1a/CDKN2a-RB-E2F信号通路诱导细胞衰老，通过下调HIF-1α和VEGF抑制血管生成，增强5-Fu的作用。血管生成；细胞凋亡；细胞衰老；结肠肿瘤；信号转导。

肿瘤发生与反复转移国际数据库描述：

Objective: To observe the anticancer effects of the granular preparation of Tenglong Buzhong decoction (,TBD), i.e Tenglong Buzhong granules (, TBG), in human SW620 colon cancer.Methods: BALB/c nude mice were subcutaneously transplanted with SW620 cells, and treated with TBG (2.56 g/kg, once per day) and/or 5-Fu (104 mg/kg, once per week) for 21 d. Apoptosis, Caspase activities and cellular senescence were measured by commercial kits. The protein expression and phosphorylation were detected by Western blot or immunohistochemistry.Results: TBG and 5-Fu inhibited tumor growth. The tumor inhibition rate of the TBG, 5-Fu, and TBG+5-Fu groups was 42.25%, 51.58%, and 76.08%, respectively. Combination of TBG and 5-Fu showed synergetic anti-cancer effects. TBG and 5-Fu induced apoptosis, activated caspase-3, -8, and -9, increased SMAC expression, inhibited XIAP expression. TBG induced cellular senescence, upregulated cyclin-dependent kinase inhibitor 1a (CDKN1a) and cyclin-dependent kinase inhibitor 2a (CDKN2a) expression, and inhibited phosphorylation of retinoblastoma-associated protein (RB) and expression of cyclin E1 (CCNE1) and cyclin-dependent kinases (CDK) 2. TBG also inhibited angiogenesis accompanied by downregulation of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α).Conclusions: TBG inhibits SW620 colon cancer growth, induces apoptosis SMAC-XIAP-Caspases signaling, induces cellular senescence through CDKN1a/CDKN2a-RB-E2F signaling, inhibits angiogenesis by down-regulation of HIF-1α and VEGF, and enhances the effects of 5-Fu.Keywords: Tenglong Buzhong granules; angiogenesis; apoptosis; cellular senescence; colonic neoplasms; signal transduction.