【佳学基因检测】恶性黑色素瘤中骨桥蛋白亚型和整合素的基因表达模式
国内正规dota2吧雷电竞 基因检测机构关键点
深究基因检测机构自我培训教材看到《Pathol Oncol Res》在. 2022 Aug 24;28:1610608.发表了一篇题目为《恶性黑色素瘤中骨桥蛋白亚型和整合素的基因表达模式》肿瘤dota2吧雷电竞 治疗基因检测临床研究文章。该研究由Krisztina Jámbor, Viktória Koroknai, Tímea Kiss, István Szász, Péter Pikó, Margit Balázs等完成。促进了肿瘤的正确治疗与个性化用药的发展,进一步强调了基因信息检测与分析的重要性。
肿瘤靶向药物及正确治疗临床研究内容关键词:
基因表达,整合素,黑色素瘤进展,骨桥蛋白,骨桥蛋白剪接变体
肿瘤靶向治疗基因检测临床应用结果
骨桥蛋白 (OPN) 是一种多功能糖蛋白,在生理上与不同类型的整合素相互作用。它被认为是某些肿瘤类型中可能的预后生物标志物;然而,存在各种剪接异构体,尚未在黑色素瘤中进行研究。我们旨在确定五种 OPN 同种型的相对表达模式,并阐明剪接变体在黑色素瘤中的预后意义。我们还旨在研究八种整合素在同一肿瘤中的表达模式。基因表达分析显示,与原发灶相比,转移性肿瘤中 OPNa、OPNb 和 OPNc 的相对表达显着高于原发灶(p < 0.01),而 OPN4 和 OPN5 在两者中的表达均较低。与表面扩散亚型相比,更具侵袭性的结节性黑色素瘤具有更高的表达水平(p ≤ 0.05)。八种测试整合素的相对表达较低,仅在结节性黑色素瘤中可检测到 ITGB3 的表达(Medianlog2 = 1.274)。 Breslow 厚度与 OPNc 变体的表达呈正相关,因此较厚的肿瘤(> 4 mm)具有显着更高的表达(p ≤ 0.05)。 Breslow厚度与OPN4的表达呈负相关,与ITGA2的表达相似。 OPNc也与转移的存在呈显着正相关。我们的数据表明,OPNa、OPNb,尤其是 OPNc 的高表达和 OPN4 和 ITGA2 的低表达与肿瘤进展的晚期和黑色素瘤的不良预后相关。整合素;黑色素瘤进展;骨桥蛋白;骨桥蛋白剪接变体。
肿瘤发生与反复转移国际数据库描述:
Osteopontin (OPN) is a multifunctional glycoprotein that physiologically interacts with different types of integrins. It is considered to be a possible prognostic biomarker in certain tumor types; however, various splicing isoforms exist, which have not been investigated in melanoma. We aimed to define the relative expression pattern of five OPN isoforms and clarify the prognostic significance of the splice variants in melanoma. We also aimed to investigate the expression pattern of eight integrins in the same tumors. Gene expression analyses revealed that the relative expression of OPNa, OPNb, and OPNc is significantly higher in metastatic tumors compared to primary lesions (p < 0.01), whereas the expression of OPN4 and OPN5 was low in both. The more aggressive nodular melanomas had higher expression levels compared to the superficial spreading subtype (p ≤ 0.05). The relative expression of the eight tested integrins was low, with only the expression of ITGB3 being detectable in nodular melanoma (Medianlog2 = 1.274). A positive correlation was found between Breslow thickness and the expression of OPNc variant, whereby thicker tumors (>4 mm) had significantly higher expression (p ≤ 0.05). The Breslow thickness was negatively correlated with the expression of OPN4, and similarly with ITGA2. OPNc also exhibited significant positive correlation with the presence of metastasis. Our data show that high expression of OPNa, OPNb, and especially OPNc and low expression of OPN4 and ITGA2 are associated with an advanced stage of tumor progression and poor prognosis in melanoma.gene expression; integrins; melanoma progression; osteopontin; osteopontin splice variants.
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